(a) The DNA fragment resolve according to their size through sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it moves. (b) The given agarose gel electrophoresis shows migration of undigested DNA fragments in lane 1 and digested set of DNA fragment in lane 2 to 4.
Why do DNA fragments migrate during electrophoresis quizlet?
Why does DNA move in electrophoresis? DNA is negatively charged so if it is in the presence of an electric current it will move toward a positive pole. Samples of DNA placed in wells cut into a horizontal slab of agarose and electrophoreses.
Where are the fragments transferred in gel electrophoresis?
A restriction enzyme is used to cut a sample of DNA into fragments that are separated using gel electrophoresis. The DNA fragments are transferred out of the gel to the surface of a membrane. The membrane is exposed to a DNA probe labeled with a radioactive or chemical tag.