EDTA Tubes (Lavender Top)
Lavender top tubes must be adequately filled to ensure the correct blood to anticoagulant ratio. Gently invert the tube 8 to 10 times immediately after collection to ensure proper mixing with the anticoagulant.
Which blood specimen does not need centrifuge?
Whole blood is obtained when blood is drawn into a tube containing an anticoagulant. The tube is mixed thoroughly and is not centrifuged. When whole blood is the required specimen, please use the following procedure: Determine the tube type needed and the required volume.
When should tubes be centrifuged?
The recommended centrifuge time is15 minutes at 3,000 rpm. All specimens collected in tubes with gel barriers should be properly centrifuged prior to transport. When gel tubes are stored after centrifugation, the serum/plasma is separated from the cells by the gel barrier.
Do you centrifuge EDTA tubes?
Plasma preparation
Collect whole blood into commercially available anticoagulant-treated tubes e.g., EDTA-treated (lavender tops) or citrate-treated (light blue tops). … Cells are removed from plasma by centrifugation for 10 minutes at 1,000–2,000 x g using a refrigerated centrifuge.
Do you spin EDTA tubes?
Pink-top tube (EDTA)
These tubes are preferred for blood bank tests. NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.
What color tube is used for sodium level test?
Red-top tube, gel-barrier tube, or green-top (lithium heparin) tube. Do not use oxalate, EDTA, or citrate plasma.
What is a tiger top tube used for?
Serum Separator Tube (SST) (Tiger Top): Does not contain an anticoagulant but contains a clot activator and serum separator gel. This tube is used for collecting serum.
What is the most critical error a phlebotomist make?
Most often these errors can be prevented by the phlebotomist following correct venipuncture procedure for every procedure, every time. Hidden errors include hemoconcentration, incorrect order of draw, and (the most serious of all errors) misidentification of patient or specimens (Table 5).
What tube does not require mixing?
4) Blood specimen tubes without additives do not require mixing. 5) It is preferable for a blood specimen tube to be maintained in a vertical position during handling after the venipuncture.
What happens if you spin blood too soon?
Failure to adhere to these wait periods may result in fibrin clots forming within the serum phase of the centrifuged sample, which may require additional handling to rim the clot and may introduce sample contamination.
Which tube does not need to be inverted?
All tubes (except red top tubes which contain no additives) must be gently inverted 5 to 8 times immediately after filling, to ensure proper mixing of blood and anticoagulant, or other additives.
What color tube is used for syphilis?
Obtain 5-10 ml of blood from the patient using standard venipuncture technique. Collect the blood into an anticoagulant free (i.e. “clot”) tube or serum separator tube (SST). Plasma (purple top tube) may be used up to 48 hrs.
Do you centrifuge gray top tubes?
Mottled red/gray-top, gold-top, or cherry red-top (gel-barrier) tube: Contains clot activator and gel for separating serum from cells, but not anticoagulant. … Serum may be sent in the centrifuge tube with an intact barrier (correct separation upon centrifugation) between cells and serum or in a plastic transport tube.
What tests are red top tubes used for?
Red top – Tube without gel separator, used mainly for serology and chemistry testing. 10mL Red top tubes are used in Blood Bank for antibody screens.
What do the different colored blood tubes mean?
The tests each bottle is used for are the same: the purple one is for cell count, the yellow one is for electrolytes, albumin and LDH, the grey one is for glucose, and blood culture bottles can be used for fluid cultures.
Which blood tube is drawn first?
The order of draw is based on CLSI Procedures and Devices for the Collection of Capillary Blood Specimens; Approved Standard – Sixth Edition, September 2008. This standard recommends that EDTA tubes be drawn first to ensure good quality specimen, followed by other additive tubes and finally, serum specimen tubes.
What color tube is CRP?
Specimen Required
Red-top tube should be centrifuged and the serum aliquoted within 2 hours of collection.
What color tube is for BMP?
Green Tube/Plasma: Centrifuge after collection. Gold Tube/Serum: Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
Why serum is preferred over plasma?
In general, serum samples (red top tubes) are preferred for chemistry testing. This is because our chemistry reference intervals are based on serum not plasma. … For example, LDH, potassium and phosphate are higher in serum than plasma, because of release of these constituents from cells during clotting.
How long can blood sit in tubes?
Tubes of blood are to be kept closed at all times. 2. Whole blood samples should not remain at room temperature longer than 8 hours. If assays are not completed within 8 hours, samples should be stored at +2°C to +8°C no longer than 7 days.
What additive is in the yellow tube?
Light yellow top tube contains the additive sodium polyanethol sulfonate (SPS). This tube is used for blood bank studies, human leukocyte antigen (HLA) phenotyping, DNA, and paternity testing.