DNA spooling is a method of obtaining DNA in a form of spool over a glass rod at the last step of DNA extraction. … This is done by inserting a thin glass rod in the tube having purified DNA, the glass rod is gently rotated with the help of fingers and DNA get adhered to it forming a spool.
Can DNA be spooled out of a solution?
DNA is soluble in water but insoluble in the presence of salt and alcohol. By gently stirring the alcohol layer with a sterile pipette, a precipitate becomes visible and can be spooled out. If there is lots of DNA, you may see a stringy, white precipitate.
Why should DNA be isolated?
The ability to extract DNA is of primary importance to studying the genetic causes of disease and for the development of diagnostics and drugs. It is also essential for carrying out forensic science, sequencing genomes, detecting bacteria and viruses in the environment and for determining paternity.