Store the saline solution in the airtight container. Research suggests that bacteria can grow in homemade saline solution within 24 hours, and that bacteria are less likely to grow when saline is chilled. Where possible, store the solution in the refrigerator.
How do you store saline solution?
Cool to room temperature before using. Saline solution can be stored at room temperature in a tightly covered glass or plastic bottle. You can keep it for up to one week. Always label it and include the date.
How long can normal saline be refrigerated?
Normal saline that is kept refrigerated may be used for a 30-day period of time. If not used within 30 days, the bottle must be discarded and a new bottle opened.